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»óÇ°ÄÚµå Description ´ÜÀ§/°¹¼ö ÆǸŰ¡ VAT ÁÖ¹®Çϱâ

Media
> Brilliant Green Bile Broth
Liquid Media
Ready-to-use media considerably reduce the preparation time in quality control laboratories and also effectively reduce the risks of cross contamination. Whatman is cooperating closely with quality assurance managers in the industry in the development of its own media and test kits.

Media
> Pseudomonas Media: Typical Growth of Pseudomonas aeroginosa ATCC 10145

This intensive product development has produced a range of products that is being used to monitor production plants and conduct microbiological checks on raw materials through to final product release in laboratories in more than 40 countries.

Media
> EC-Broth: Vial left: Control; Vial right: Broth Inoculated with Escherichia coli ATCC 25922

Features and Benefits
  • Wide range of products satisfies even special customer requirements
  • Optimal media stability, sterility and reproducibility
  • Less time-consuming, higher productivity
  • Manufacturing process and product checks according to the requirements of pharmacopoeia throughout the world
  • Batch-specific quality certificate in each pack



Media
> 2mL Ampouled Media

Media variety and flexibility
Do you use ready-to-use media in 2 mL ampoules? Are you looking for nutrient media or agar plates that are specifically suited to the cultivation of colonies after microfiltration? Would you perhaps like to fill your own agar plates? No matter what your specific needs, our extensive range of ready-to-use or prepared media products is available for just about any application.

Media
> MI-Media: Pure Culture of Escherichia coli ATCC 25922 with UV Light

Liquid media products come with a host of advantages, too. Aseptic preparation of nutrient media saves you valuable time and keeps costs to a minimum. All media undergo detailed quality control checks in accordance with recognized methods, guaranteeing uniform media preparation at all times. Lastly, our more comprehensive end product tests ensure optimal growth as well as stable and sterile media.

Media
> M-Green Yeast & Mold Agar: Typical Fungal Growth on Agar with White Membrane Filter

Whatman goes to great lengths to ensure that all legal regulations are met, much the same way we observe the standards of the pharmaceutical industry. We put this assurance in writing, too. Each package is accompanied by a batch-specific quality certificate.

Dilution Bottles
Our pre-filled and sterile dilution vials are designed for sample dilution of water, dairy products, foods, and pharmaceuticals prior to microbiological testing. Final pH for all solutions is 7.2 pH ¡¾ 0.2 pH at 25¡Æ C. They come in an easy open, flip-top container with a tamper-evident seal.
Butterfield¡¯s Phosphate Buffer contains monobasic potassium phosphate and is used extensively in the food dairy and pharmaceutical industries. Offered in 90 mL and 99 mL volumes for easy 1:10 dilutions. It is recommended as a general diluent in laboratory procedures by the Federal Drug Administration and in the Bacteriological Analytical Manual. This product is prepared according to Standard Methods for the Examination of Water and Wastewater for use in water testing.
Phosphate Buffer with Magnesium Chloride is used as the diluent for the preparation of dilutions in plate counts in the dairy and food industries. It is recommended by the APHA for the recovery of injured microorganisms from dairy and food samples. Contains deionized water, monopotassium phosphate, and magnesium chloride.

Media Descriptions
Brilliant Green Bile Broth 2%
BGBB contains two inhibitors of both gram-positive and selected gram-negative organisms, namely, oxgall and brilliant green dye. Fermentation is detected by gas production.
Cetrimide Broth
Pseudomonas aeruginosa is characterized by the production of pyocyanin (a blue green, water soluble, nonfluorescent, phenazine pigment), which is stimulated by the inclusion of magnesium chloride and potassium sulfate in the broth.
Cetrimide (N-cetyl-NNN-trimethylammonium bromide) is added to inhibit bacteria other than Pseudomonas aeruginosa. Its action as a quaternary ammonium cationic detergent causes nitrogen and phosphorous to be released from bacterial cells other than Pseudomonas aeruginosa.
EC Broth
EC Broth contains casein peptone as a source of nutrients. Lactose provides the carbohydrate fermented by coliform bacteria and Escherichia coli. In addition, lactose-positive bacteria metabolize lactose with gas formation. Gram-positive bacteria are inhibited by the mixture of bile salts.
EC Broth with MUG
The presence of the fluorescence using a long-wave UV light source confirms the presence of Escherichia coli, and no further confirmation is required.
MUG detects anaerogenic strains, which may not be detected in the conventional procedure. Lactose is a source of energy. Casein peptone provides additional nutrients. The mixture of bile salts is inhibiting for gram-positive bacteria, particularly bacilli and fecal streptococci. The substrate 4-methylumbelliferyl-©¬-D-glucuronide is hydrolyzed by an enzyme, ©¬-glucuronidase, possessed by most Escherichia coli and a few strains of Salmonella, Shigella and Yersinia, to produce a fluorescent end product, 4-methylumbelliferone.
Enterococcus Broth
Enterococcus broth is a modified version of the improved media described by Slanetz and Bartley with TTC. The membrane filtration method is simple to perform, does not require confirmation and permits a direct count of enterococci in 48 hours.
Eugon Broth
Eugon media was developed to obtain eugonic (luxuriant) growth of fastidious microorganisms. The unenriched media supports rapid growth of lactobacilli associated with cured meat products, dairy products and other food. The high concentration of Dextrose is the energy source for rapid growth of bacteria. L-cystine and sodium sulfite are added to stimulate growth. Sodium chloride maintains the osmotic balance of the media. The high carbohydrate content along with high sulfur (cystine) content improves growth with chromogenicity.
HPC Broth with TTC
HPC is used to determine total count at incubation temperatures of 35¨¬ C. All bacteria develop on HPC with indicator media and produce a red color as a result of the precipitation of formazan following the reduction of 2,3,5- triphenyltetrazolium chloride (TTC) by bacteria.
KF-Streptococcus Broth
KF-Streptococcus Broth is selective for the determination of fecal streptococci in polluted surface waters. Maltose and lactose are fermentable carbohydrates, sodium azide is the selective agent and brom cresol purple is the indicator dye.
Lauryl Sulfate or Lauryl Tryptose Broth
This media was developed for the detection of coliform organisms by the American Public Health Association (APHA). It is now the standard medium of choice in the presumptive phase of the standard coliform MPN test for the microbiological examination of water.
Mannitol Salt Broth
Because of the amount of peptones and beef extract, Mannitol Salt is a nutrient rich medium. Most bacteria (other than staphylococci) are inhibited by the high concentration of sodium chloride. Organisms capable of fermenting mannitol, e.g. Staphylococcus aureus, cause a pH change in the media. With phenol red as the pH indicator the colonies appear with a yellow coloration.
Membrane Lauryl Sulfate Broth
This media was developed for the detection of coliform organisms and is now the media of choice for the enumeration of total coliforms and Escherichia coli in the United Kingdom. This media replaced membrane enriched broth containing 0.4% Teepol 610.
M-Endo Coliform Broth
M-Endo is a red colored media, which needs to be stored in the dark to prevent discoloration of the media. Gram-positive bacteria are inhibited on this media by the desoxycholate and lauryl sulfate. The addition of ethanol increases the antibacterial nature of the formulation. Lactose fermenting organisms form aldehydes, which react with Schiff's reagent (basic fuchsin and sodium sulfite) to give red colored zones around the colonies. Coliform colonies are therefore red with a characteristic metallic sheen.
M-FC Broth
Allows the development of fecal coliforms at elevated temperatures (44.5¡Æ C).
M-FC with Rosolic Acid
M-FC with Rosolic Acid acts and functions in the same way as m-FC Broth. Rosolic acid inhibits bacterial growth in general, except for fecal coliforms.
M-Green Select Broth
M-Green Select Broth is an improved modification of the liquid media, m-Green Yeast and Mold Broth and was developed to improve efficiency of detection and enumeration of fungi in sugar based drinks using the membrane filtration method. This medium has a low pH, which inhibits bacterial growth. The addition of Chloramphenicol further inhibits the growth of bacteria to allow for the development and enumeration of yeast and mold. The addition of bromocresol green, which diffuses into fungal colonies as an alkaline reaction, allows them to be easily identified. Metabolic by-products from the developing colonies diffuse into the surrounding medium, further reducing the pH which aids in the inhibition of bacterial growth, but also produces an acid reaction that causes residual bromocresol green to change to yellow.
M-Green Yeast and Mold
M-Green is an improved modification of the liquid media, m-Yeast and Mold Broth and was developed to improve efficiency of detection and enumeration of fungi in sugar based drinks using the membrane filtration method. This medium has a low pH, which inhibits bacterial growth. The addition of bromocresol green, which diffuses into fungal colonies as an alkaline reaction, allows them to be easily identified. Metabolic by-products from the developing colonies diffuse into the surrounding medium, further reducing the pH which aids in the inhibition of bacterial growth, but also produces an acid reaction that causes residual bromocresol green to change to yellow.
MI Broth and MI Agar
MI Broth detects the presence of coliform bacteria by the production of ©¬-galactosidase, which cleaves the substrate MUGal to produce 4-Methylumbelliferone, which fluoresces on exposure to UV light. Non-coliforms do not produce this enzyme and therefore do not fluoresce on the medium. Escherichia coli is detected by the compound IBDG. The ©¬-glucuronidase produced by Escherichia coli cleaves the substrate to produce a blue indigo color in the colonies. As Escherichia coli is also a total coliform, and also produces ©¬-galactosidase, it will also fluoresce. The antibiotic cefsulodin is added to inhibit the growth of gram-positive bacteria and some non-coliform gram-negative bacteria that can cause false positive reactions.
MRS Broth
MRS medium supports luxuriant growth of all lactobacilli, even the slow growing species.
M–TGE Total Count Media
All bacteria develop on TGE media and produce a range of different colored and sized colonies.
Orange Serum Media
Organisms known to grow in single strength and concentrated juices are lactic acid and acetic acid bacteria and yeast. Lactobacilli, Leuconostoc and yeast have all been identified as spoilage organisms by numerous authors. Orange serum at pH 5.4 to 5.6 has been reported to yield maximum counts of all types of spoilage organisms in mixed cultures, and in single culture comparison tests.
Potato Dextrose Broth and Agar Media
Potato Dextrose Broth is recommended in Standard Methods as the media that gives the most consistent and highest counts for the recoveries of yeast and mold in dairy products. The inclusion of potato extract encourages the growth and development of fungi. Sterile tartaric acid may be added to lower the pH to 3.5 ¡¾ 0.2 to further inhibit the growth of conflicting bacteria.
Pseudomonas Broth
Pseudomonas aeruginosa is characterised by the production of pyocyanin (a blue green, water soluble, non-fluorescent, phenazine pigment), which is stimulated by the inclusion of magnesium chloride and potassium sulfate in the broth. Irgasan, an antimicrobial agent, selectively inhibits gram-positive and gram-negative bacteria other than pseudomonads. Glycerol serves as both an energy source and helps in the promotion of pyocyanin.
R2 Broth
R2 broth can be used to determine heterotrophic plate count at 35¨¬ C. When incubated at lower temperatures (25–30¨¬ C) for longer periods of 72–96 hours it can also be used to recover environmentally stressed organisms, or those that are chlorine tolerant.
Sabouraud Dextrose Broth
Peptone in the media is used as a nitrogen source for the development of fungi. Dextrose acts as an energy source for the growth of microorganisms. The low pH is favorable for the development of fungi, especially dermatophytes, but at the same time inhibits the development of contaminating bacteria from clinical specimens.
Standard Methods Agar
All bacteria develop on Standard Methods and produce a range of different colored and sized colonies.
Total Count Media with TTC
All bacteria develop on Total Count Media with indicator and produce a red color as a result of the precipitation of formazan following the reduction of 2,3,5-triphenyltetrazolium chloride (TTC) by bacteria.
Trypticase Soy Broth – Single Strength
General-purpose medium used in qualitative procedures for the cultivation of fastidious and non-fastidious microorganisms. Trypticase Soy Broth – Single Strength complies with the demands of the DIN Norm 10167 for the detection of Escherichia coli serotype 0157:H7 in foods and FDA-BAM for the isolation of enterohemorrhagic Escherichia coli (EHEC). In addition the media conforms to the formula of the U.S. Pharmacopoeia.
Trypticase Soy Broth – Double Strength
TSB broth is a medium that will support the growth of a wide variety of microorganisms including aerobic, facultative and anaerobic bacteria and fungi.
Wallerstein Nutrient Broth (WL) and WLDifferential Broth (WLD)
Use of the medium at pH 5.5 and incubation at 25¡Æ C will give reliable counts for brewer¡¯s yeast. Adjustment of the pH to 6.5 and incubation at 30¡Æ C allows for the selective growth of baker's and distiller's yeast.
Ordering Information - Liquid Media
Description Use Quantity/ Pack Catalog Number
2 mL Ampoules
Cetrimide Broth Pseudomonas aeruginosa 50 10 496 146
Enterococcus Broth Isolation and enumeration of enterococci 50 10 496 120
Eugon Broth Wide variety of microorganisms 50 10 496 126
HPC Broth with TTC Heterotrophic plate counts 50 10 496 151
KF-Streptococcus Broth Isolation and enumeration of fecal streptococci 50 10 496 125
Mannitol Salt Broth Selective isolation and enumeration of staphylococci 50 10 496 121
Membrane Lauryl Sulfate Broth Presumptive identification of coliforms and E.coli 50 10 496 187
M-Endo Coliform Broth Enumeration of coliforms 50 10 496 103
M-FC Broth Coliform detection in water pollution 50 10 496 124
M-FC Broth with Rosolic Acid Fecal coliform detection 50 10 496 114
M-Green Select Broth Enumeration of yeasts and mold in soft drinks and fruit juices 50 10 496 116
M-Green Yeast and Mold Enumeration of yeast and mold in soft drinks and fruit juices 50 10 496 101
MI Broth Coliform detection according to Surface Water Treatment Rule (USEPA)
and Total Colifrom Rule (USEPA)
50 10 496 192
MRS Broth Isolation and cultivation of Lactobacilli 50 10 496 112
M-TGE Total Count Media Non-selective development and enumeration of all aerobic bacteria 50 10 496 102
Orange Serum Media Acid-tolerant microorganisms 50 10 496 104
Pseudomonas Broth Isolation of Pseudomonas 50 10 496 119
R2 Broth Heterotrophic plate counts 50 10 496 161
Total Count TTC Indicator Colony count 50 10 496 113
Wallerstein Nutrient Broth (WL) Cultivation and enumeration of yeast 50 10 496 108
WL Differential Broth (WLD) Bacterial counts 50 10 496 109
Liquid Media in 9 mL Tubes
Brilliant Green Bile Broth 2% Coliform detection 20 10 496 710
EC Broth Coliform detection @ 37¡Æ C and E. coli @ 44.5¡Æ C 20 10 496 714
EC Broth with MUG E. coli in water and food samples by fluorogenic procedure 20 10 496 709
Lauryl Sulfate/Tryptose Broth Coliform detection 20 10 496 722
Bottled Media
M-Endo Coliform Broth - 50 mL Coliforms 8 10 496 700
MI Broth Coliform detection according to Surface Water Treatment Rule (USEPA)
and Total Colifrom Rule (USEPA)
50 10 496 851
MI Agar - 50 mL Coliform detection 1 10 496 847
Orange Serum Agar - 100 mL Acid-tolerant microorganisms 1 10 496 713
Potato Dextrose Agar - 100 mL Cultivation and enumeration of yeast and mold 1 10 496 731
Standard Methods Agar - 100 mL Microbiological plate counts 1 10 496 706
Trypticase Soy Broth
Single Strength - 100 mL
Cultivation of fastidious and non-fastidious microorganisms 1 10 496 707
Trypticase Soy Broth
Double Strength - 100 mL
Cultivation of fastidious and non-fastidious microorganisms 1 10 496 708
Dilution Bottles
Butterfield's Buffer Potassium Phosphate 99 mL 10 498 503
Butterfield's Buffer Potassium Phosphate 90 mL 10 498 504
Phosphate Buffer Potassium Phosphate with Magnesium Chloride 99 mL 10 498 505
Petri Dishes
Petri Dishes with Sterile Pads 47 mm diameter 100 10 498 544
Petri Dishes with Sterile Pads 50 mm diameter 50 10 445 905



Related documents for download 
  •  Media Selection Guide
  •  MI Broth and Other Media Data Sheet
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