■ 제품코드(CAT No) / 가격표(Price)
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CAT No. |
Description |
단위/갯수 |
Price[VAT별도] |
상품문의 |
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규격외 주문제작 및 가격 내고 가 필요로 하신 분은 별도로 견적요청 or 전화문의 바랍니다. FAX:02)967-5979
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■ 용도(Applications)
■특 징 (Features )
For Cell Disruption we suggest:
* When working with Bacteria use the 0.1mm diameter glass beads.
* When working with Yeast/Fungi use the 0.5mm diameter glass beads.
* When working with Most Tissue use the 1.0mm diameter glass beads or zirconia/silica beads.
* When working with Skin or "soft" plant material use a 2.0mm diameter zirconia beads. * When working with really tough or fibrous tissue use the same sized beads (see above) but choose a more dense bead material.
* For example, researchers prefer 0.1mm zirconia-silica beads for disruption of spores or 2.3 mm chrome-steel beads for extraction of tough fibrous plant material like monocot leaves.
* Some users have had good results using the MiniBeadbeater in a "dry grinding" process at liquid nitrogen temperatures.........
* Feed Back, Oct 2006. Santhosh Chelian of UC Davis. Fresh rice leaves or tree needles were powdered or pulverized with a MiniBeadbeater using several 2.5 mm or
3.2 mm chrome steel beads in a microvial. The capped vial contents were immersed 1/2 way into liq. N2 and and then quickly inserted into a MBB. No liq. N2 is added
to the vial. Pulverize for one minute. Refreeze and repeat for another 1/2 min, if necessary. 40-60 mg of plant material was used in each well. (BIOSPEC NOTE: Do
not use 6.35 mm steel beads for dry milling in our MBB machines, as they will blast right through the vials! Glass beads of the same 6.35 mm size, however, are
okay. For MiniBeadbeater-96 users, the same cryogenic protocol works using 12 strips of 1.2 ml micro dilution tubes (96 well format, USA Scientific, #1212-8000).
Also, consider using the solid aluminum vial holder, Cat. No. 702ALU, chilled to liq. N2 temperatures, with an array of individual microvials in the MBB-96).
* After cryo-pulverizing the above tissue, DNA or RNA extraction solution was added to the vial and the mixture was "beadbeat" for an additional 1-3 minutes at room
temperature.
* Nucleic acids were recovered in high yield. (BIOSPEC NOTE: Cryo-pulverized tissue, gently extracted with DNA extraction solution with no further physical homogenization, yields very large molecular weight DNA.)
* Freeze-dried leaf tissue can also be dry ground. In this case, cryo-temperatures are not needed during grinding.
* Use a few 3.5mm or two 6.35mm glass beads per well or vial and grind for 3-5 minutes.
* Preliminary information suggests that plant and animal tissue can be disrupted more quickly with sharp-edged material rather than smooth beads.
* Furthermore, it may not be necessary to prechop larger sized tissue into small pieces before "beadbeating". If you want to check this out, we now stock three sizes of Silicon Carbide sharp particles.
* Feedback, Mar 2006. James Brien of Health & Science, University in Portland, OR.
* Brien reports that several whole organs from mice, cut into 3-4 pieces, were completely homogenized using 1 mm dia. SiC sharp particles.
* His objective was to measure virus load using a plaque assay.
* He found that the usual prechopping of the tissue to 1mm cross-section pieces was not necessary. Using a dye permeability test, he estimated that better than 99% of the cells were lysed after 2 minutes of
* shaking in a MiniBeadbeater-96. Lysis in both 2 ml and 7 ml vials was tested. One half of the vial volume was filled with the sharp particles. Controls using similar loads
of 1 mm dia. spherical glass beads gave poorer results.
* Coating beads is a waste of time.
* The coating will be quickly removed during shaking. Most bead media is reusable. Wash with lab detergent (not acid!) and rinse well (see Bead Washing link below).
Other uses for beads:
* Quick and easy plating of yeast and bacteria. Add a few sterile 6.3mm diameter glass beads to the plate to evenly spread yeast and bacteria inoculums. No need to remove them afterward.
* Pack roller bottles, tubes or vials with 6.3 mm diameter glass beads to greatly increase the surface area for tissue culture growth. In this application the beads should be packed tightly to prevent movement of the beads during rolling or shaking. * When growing cell in non-agitated culture flasks, confluence is reached faster and cell density is enhanced by adding a layer of 0.1 mm diameter beads (see Growth of Three Established Cell Lines on Glass Microcarriers by James Carani, et. al., Biotechnology and Bioengineering, Vol.25,
p.1359-1372 (1983).
* Create a bio-reactor by packing a column with glass beads and inoculate with select surface-adhering micro-organisms or cells.
* Keep dialysis tubes vertical during dialysis by adding a few large beads before sealing.
Density - - -
* Glass has a density of 2.5 g/cc (most commonly used bead media for "Beadbeating")
* Zirconia/Silica has a density of 3.7g/cc (50% more dense than glass - good for spores and most tissues)
* Silicon Carbide (sharp particle, not a bead) has a density of 3.2 g/cc (May work faster on tissue samples because the particles have sharp cutting edges. Their utility is still under investigation, but see Brein's comments above)
* Zirconia has a density of 5.5g/cc (100% more dense than glass - good for tough tissue)
* Steel, stainless or not, has a density of 7.9g/cc (used mostly for grinding leaves - use chrome steel beads one time, as unlike stainless steel, they not rust-proof. Chrome steel beads are about 10X cheaper)
* Tungsten Carbide has a density of 14.9g/cc (While very dense, this bead is not recommended for biopreps - leaves prep dirty)
■ 규격(Specifications)
Ordering Information - - -Buy beads (one lb bottle) and load the vials yourself or order pre-loaded vials (45 vials/pkg)
(Note that bead sizes given below are median values. Sorted by sieves, actual bead size will range +/-10% of the average value)
Cat. No. 11079101 Glass Beads, 0.1mm dia $29 for 1 lb.(454g)
Cat. No. 11079105 Glass Beads, 0.5mm dia $29 for 1 lb.(454g)
Cat. No. 11079CUST-05G‡ One cc of 0.5 mm Glass Beads in two ml polypropylene, screw-cap vials. 45 in a polybag for $60.
Cat. No. 11079110 Glass Beads, 1.0mm dia $29 for 1 lb.(454g)
Cat. No. 11079125 Glass Beads, 2.5mm dia $29 for 1 lb.(454g)
Cat. No. 11079135 Glass Beads, 3.5mm dia $29 for 1 lb.(454g)
Cat. No. 11079635 Glass Beads, 6.4mm dia $29 for 1 lb.(454g)
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‡ Several more preloaded vial combinations are being planned - let us what
you want at info@biospec.com.
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Cat. No. 11079101z Zirconia/Silica, 0.1mm $42 for 1 lb.(454g)
Cat. No. 11079105z Zirconia/Silica, 0.5mm $42 for 1 lb.(454g)
Cat. No. 11079110z Zirconia/Silica, 1.0mm $42 for 1 lb.(454g)
Cat. No. 11079125z Zirconia/Silica, 2.3mm $42 for 1 lb.(454g)
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Cat. No. 11079107zx Zirconia, 0.7mm $75 for 1 lb.(454g)
Cat. No. 11079110zx Zirconia, 1.0mm $75 for 1 lb.(454g)
Cat. No. 11079124zx Zirconia, 2.0mm $75 for 1 lb.(454g)
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Cat. No. 11079112ss Stainless-Steel, 1.2mm $39 for 45g (not 1 lb.!)
Cat. No. 11079123ss Stainless-Steel, 2.3mm $39 for 90g (not 1 lb.!)
Cat. No. 11079132ss Stainless-Steel, 3.2mm $39 for 90g (not 1 lb.!)
Cat. No. 11079635ss Stainless-Steel, 6.35mm $39 for 100 beads
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Cat. No. 11079113c Chrome-Steel, 1.3mm $54 for 1 lb.(454g)
Cat. No. 11079123c Chrome-Steel, 2.3mm $54 for 1 lb.(454g)
Cat. No. 11079132c Chrome-Steel, 3.2mm $27 for 1 lb.(454g)
Cat. No. 11079635c Chrome-Steel, 6.35mm $27 for 1 lb.(454g)
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Cat. No. 11079111wc Tungsten-Carbide, 1.1mm $62 for 90g*
* Note: These beads impart a brown-gray color to homogenates. This inert, colloidal tungsten-carbide can often be removed by centrifugation or filtration.
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Cat. No. 11079101sc Silicone-Carbide Sharp Particles, 0.1mm $35 for 1 pound (454g) **
Cat. No. 11079110sc Silicone-Carbide Sharp Particles, 1mm $35 for 1 pound (454g) **
Cat. No. 11079140sc Silicone-Carbide Sharp Particles, 4 mm (irregular, rectangular shape) $35 for 1 pound (454g)**
** Note: The very sharp, hard edges of these particles impart knife-like cutting action during high-energy shaking that may abrade and micro-chop tough tissue samples faster than beads. Wash with lab detergent before first use (see Cleaning Beads link below). |
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